98% BTM BIS Tris Buffer Biological CAS 6976-37-0 Molecular Biology
BIS-Tris CAS 6976-37-0 Biological Buffer 98% BTM
| 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol Basic information |
| Product Name: | 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol |
| Synonyms: | 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE;2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL;2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol;Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane;Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade;Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS;Bis-Tris (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol);BIS-TRIS BIOULTRA, >= 99.0% (NT) |
| CAS: | 6976-37-0 |
| MF: | C8H19NO5 |
| MW: | 209.24 |
| EI NECS: | 230-237-7 |
| Product Categories: | Buffer;API intermediates |
| Mol File: | 6976-37-0.mol |
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| 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol Chemical Properties |
| Melting point | 104 °C(lit.) |
| Boiling point | 466.2±40.0 °C(Predicted) |
| density | 1.346±0.06 g/cm3(Predicted) |
| storage temp. | Inert atmosphere,Room Temperature |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | crystalline powder |
| color | whiet |
| Odor | Odorless |
| PH | 9.0-10.1 (20-25℃, 1M in water) |
| PH Range | 5.8 - 7.2 |
| pka | 6.5(at 25℃) |
| Water Solubility | soluble |
| λmax | λ: 280 nm Amax: 0.15 |
| Sensitive | Air Sensitive |
| BRN | 2205275 |
| InChIKey | OWMVSZAMULFTJU-UHFFFAOYSA-N |
| 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol Usage And Synthesis |
| Chemical Properties | White/clear crystalline powder |
| Uses | It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin. |
| Uses | Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.
Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations. |
| Purification Methods | Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in "Amino Acids and Peptides", Chapter 6. |
