DTT CAS 3483-12-3 DL-Dithiothreitol Biochemical Reagents Powder
DTT CAS 3483-12-3 DL-Dithiothreitol Biochemical Reagents Powder
Description
Name:Form:Color:Storage Temp:Reaction Suitability:Samples:Package:Assay:High Light:
| DL-Dithiothreitol | Powder |
|
| White | 2-8°C |
|
| Reagent Type: Reductant | Available |
|
| Bottle/Poly Bottle/Drum | 98% |
|
3483-12-3 DL-Dithiothreitol, DL-Dithiothreitol Biochemical Reagents Powder |
DTT CAS 3483-12-3 DL-Dithiothreitol Biochemical Reagents Powder
| DL-Dithiothreitol Basic information |
| Product Name: | DL-Dithiothreitol |
| CAS: | 3483-12-3 |
| MF: | C4H10O2S2 |
| MW: | 154.25 |
| EIN ECS: | 222-468-7 |
| Mol File: | 3483-12-3.mol |
|
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| DL-Dithiothreitol Chemical Properties |
| Melting point | 41-44 °C(lit.) |
| alpha | -0.2～+0.2°(20℃/D)(c=5,H2O) |
| Boiling point | 125 °C |
| density | 1.04 g/mL at 20 °C |
| vapor density | 5.3 (vs air) |
| refractive index | 1.5200 (estimate) |
| Fp | >230 °F |
| storage temp. | -20°C (or 4°C short term). |
| solubility | H2O: 50 mg/mL, clear, colorless |
| pka | pK1:8.9 (25°C) |
| form | Powder |
| color | White |
| Odor | Unpleasant Odor |
| PH | 4.0-6.0 (20-25℃, 0.1m in H2O) |
| PH Range | 4 - 6 at 15,4 g/l at 25 °C |
| Water Solubility | freely soluble |
| λmax | λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100 |
| Sensitive | Air Sensitive |
| Merck | 14,3376 |
| BRN | 1719757 |
| Stability: | Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C. |
| InChIKey | VHJLVAABSRFDPM-UHFFFAOYSA-N |
| CAS DataBase Reference | 3483-12-3(CAS DataBase Reference) |
| NIST Chemistry Reference | 2,3-Butanediol, 1,4-dimercapto-, (r*,r*)-(3483-12-3) |
| EPA Substance Registry System | 1,4-Dithiothreitol (3483-12-3) |
| DL-Dithiothreitol Usage And Synthesis |
| Chemical Properties | White solid |
| Uses | Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins. |
| Uses | DTT is a cell permeable protective agent for Thiol groups. |
| Purification Methods | Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.] |
General Description | An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). |
