Protein A Affinity Columns For Monoclonal Antibody Analysis
Absolut A designed for fast analysis of monoclonal antibody (mAb) concentration (titer) in Affinity Chromatography. Recombinant protein A (rProtein A) has the advantages of bind the Fc region of immunoglobulins and alkali resistance. The spherical PS/DVB (Poly(styrene/divinylbenzene)) particles are highly cross-linked for enhanced mechanical stability, and bigger hole diameter. Compared with agarose base, hydrophilic PS/DVB has a higher dynamic binding capacity (DBC), longer lifetime and less shedding of rProtein A ligand. Hence, Absolut A is an excellent choice for mAbs analysis.
Advantages
• Direct use on HPLC instruments
• High dynamic binding capacity, quick mass transfer
• Minimum nonspecific absorption, accurate determination
• Fast analysis cycle time: 2-5 minutes
• Satisfactory linearity in wide concentration range: 0.02-10 mg/ml
• Long lifetime: approx. 3000 analysis cycles
• Alkali resistance: 0.1-0.5 M NaOH cleaning conditions
Main Characteristics
Column Size: 2.0mm D×30mm L, 2.1mm D x100mm L, 4.6mm Dx50mm L, 4.6 mm D x100mm L
Support Matrix: Poly(styrene/divinylbenzene) (PS/DVB)
Ligand: Recombinant Protein A
Particle Size: 30µm
Dynamic Capacity (DBC): Approx. 30 mg human IgG/ml media
Shipping Solution: 0.02 M sodium phosphate, pH 7.0, 0.02% sodium azide
pH range: pH 2-10
Maximum Operating Pressure: 1200 psi
Cleaning Agents: 0.1-0.5M NaOH
Cycle Time: 2-5 minutes
Lifetime: About 3000 analysis cycles
Temperature Stability: 4-40 °C
