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Chymotrypsin proteomics grade / chymotrypsin for peptide mass fingerprinting

Place of Origin: Zhejiang

Company Profile

Location: Shanghai, China (Mainland)
Business Type: Manufacturer
Main Products: Recombinant Trypsin, Sequencing Grade Trypsin, Recombinant Carboxypeptidase B

Product Detail

Model No.: SRCT03

Product Description

Chymotrypsin proteomics grade / chymotrypsin for peptide mass fingerprinting

 

Sequencing Grade Chymotrypsin / Chymotrypsin / SRCT / sequencing grade enzyme

 

DESCRIPTION

Sequencing Grade Trypsin

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene

 sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl

 side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at

 Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. 

 

MAIN FEATURES

 

1. Source                E. Coli

2. Purified by             HPLC 

3. Physical form          Lyophilized powder

4. Specific activity        NLT 1500 USP units/mg pro

5. Contaminant activity   No carboxypeptidase A, or other proteases contaminant.

 

APPLICATION

 

Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or

 MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

 

RECOMMEND USAGE

 

To prepare 1-10mg/ml with 1ml 1mM HCl, used within 2 days, or stored below -20 after repacked 

 

RELATED PRODUCT

 

Recombinant Trypsin (sequencing purity);

Recombinant Carboxypeptidase B (sequencing purity); 

Recombinant Chymotrypsin (sequencing purity);

V8 (sequencing purity).

 

Web:http://www.yaxinbio.com Email:Zhaozhi@yaxinbio.com

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