High purity Recombinant Carboxypeptidase B is used for producing recombinant Polypeptide
| Place of Origin: | Zhejiang |
|---|
Company Profile
| Location: | Shanghai, China (Mainland) |
|---|---|
| Business Type: | Manufacturer |
| Main Products: | Recombinant Trypsin, Sequencing Grade Trypsin, Recombinant Carboxypeptidase B |
Product Detail
| Model No.: | RCPB01 |
|---|
Product Description
Recombinant Carboxypeptidase B is used for producing recombinant Polypeptide
Recombinant Carboxypeptidase B / RCPB / Carboxypeptidase B / High purity
DESCRIPTION
Recombinant Carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the
C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is
6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by
metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B (EC 3.4.17.2) is expressed in E.Coli
and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as
carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the
preparation.
MAIN FEATURES
|
Source |
E. Coli |
|
Purified by |
HPLC |
|
Physical form |
White or white-like lyophilized powder |
|
Additives |
Tris salts, NaCl salts and carbohydrates |
|
Protein content |
35% 50% |
|
Specific activity |
≥120 units/mg pro.; ≥170 units/mg pro |
|
Purity |
> 95% |
|
Contaminant activity |
No chymotrypsin,carboxypeptidase A,and other protease contaminant. Less than 10 ppm of recombinant trypsin. |
PRODUCT INFORMATION
Product : Recombinant Carboxypeptidase B
Cat. No. : RCPB01
Specific activity : ≥170 u/mg pro
Packaging : 10mg,100mg,1g,bulk
Manufacture : YaxinBio
UNIT DEFINITION:One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25, pH 7.65.
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