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For Enzymatic Hydrolysis of ProteinCAS 9002-07-7 / Protein sequencing

Place of Origin: Zhejiang

Company Profile

Location: Shanghai, China (Mainland)
Business Type: Manufacturer
Main Products: Recombinant Trypsin, Sequencing Grade Trypsin, Recombinant Carboxypeptidase B

Product Detail

Model No.: RPT0201

Product Description

for Enzymatic Hydrolysis of Protein,CAS:9002-07-7,Protein sequencing

 

 

Bulk Recombinant Trypsin For Manufacturing Recombinant Insulin

 

DESCRIPTION

Recombinant Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as from carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

 

ADVANTAGES


Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stable:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1. Recombinant porcine trypsin provides increased specificity and eliminates contaminating activities found in enzymes with lower purity level.
2. No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3. One single main band at 24kD by SDS-PAGE.

 

MAIN FEATURES 

 

Source

E. Coli

Purified by

HPLC

Physical form

White lyophilized powder

Additives

Carbohydrates

Protein content

35%  70%

Specific activity

≥3800 USP units/mg pro

Purity

> 99%

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.

 

PRODUCT INFORMATION

 

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a 

                             reaction volume of 3.0ml at pH7.6 and 25, with BAEE as a substrate (1cm light path).

 

Product

Cat. No.

Specific activity

Packaging

Manufacturer

Recombinant Porcine Trypsin

RPT0201

≥3800 USP u/mg pro

10mg,100mg,1g or bulk

YaxinBio

 

RELATED PRODUCT

 

Recombinant Carboxypeptidase B

 

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