For Enzymatic Hydrolysis of ProteinCAS 9002-07-7 / Protein sequencing

for Enzymatic Hydrolysis of Protein,CAS:9002-07-7,Protein sequencing

Bulk Recombinant Trypsin For Manufacturing Recombinant Insulin

DESCRIPTION

Recombinant Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as from carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

ADVANTAGES

Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.
Stable:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1. Recombinant porcine trypsin provides increased specificity and eliminates contaminating activities found in enzymes with lower purity level.
2. No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3. One single main band at 24kD by SDS-PAGE.

MAIN FEATURES 

PRODUCT INFORMATION

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a 

                             reaction volume of 3.0ml at pH7.6 and 25, with BAEE as a substrate (1cm light path).

RELATED PRODUCT

Recombinant Carboxypeptidase B