HBsAg ELISA KIT

Min. Order:	100 Bag/Bags
Payment Terms:	T/T
Supply Ability:	1000

Company Profile

Daan Gene Co., Ltd. Of Sun Yat-Sen University

Location:	China (Mainland)
Business Type:	Manufacturer, Trading Company

Product Detail

Means of Transport:	Air
Brand Name:	DAAN
Production Capacity:	1000
Packing:	usually we use our package. if customer have other requirement,
Delivery Date:	within 15 days after paymentusually

Show

Product Description

Advantage

1.Certificate:ISO13485:2003, GMP
2.Sensitivity>9/10
3.Specificity>14/15
4.Precision: CV<15%

INTEND USED

Anti-HIV1+2 is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of antibodies to human immunodeficiency virus type 1 and/or 2 in human serum or plasma.

DAAN HBsAg ELISA is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of Hepatitis B Surface Antigen(HBsAg) in human serum or plasma.

INTRODUCTION

Sensitive enzyme immunoassays for the detection of HBsAg were first described by Engvall and Perlmann and VanWeemen and Schuurs in 1971. In 1976 and 1977, solid phase "sandwich" enzyme immunoassays were developed in which HBsAg was captured on a solid phase coated with polyclonal antibodies against HBsAg (anti-HBs) and then detected with anti-HBs conjugated to an enzyme. In recent years, monoclonal anti-HBs assays have been developed for the detection of HBsAg.

PRINCIPLES OF THE PROCEDURE

The wells of the polystyrene microplate strips are coated with mouse monoclonal anti-HBs. Both human serum or plasma and polyclonal anti-HBs labeled with horseradish peroxidase are incubated in these coated wells. HBsAg, if present, will bind to the solid phase antibody and the labeled antibody resulting in the HBsAg being sandwiched between the solid phase antibody and labeled antibody. Excess unbound HBsAg and labeled antibodies are removed by washing. Substrate solution containing hydrogen peroxide and 3,3',5,5'-tetramethyl- benzidine(TMB) is then added to each well. The presence of HBsAg is indicated by the presence of a blue color after substrate addition. Reaction is terminated by addition of sulphuric acid. The intensity of the color is measured spectrophotometrically at 450nm and is proportional to the amount of HBsAg present in the specimen.

KIT COMPONENTS

Microplate 1 plate (96 wells). 8 strips per plate, each with 12 wells coated with anti-HBs.
Positive Control 1 vial (1.0ml). Inactivated Human serum containing HBsAg. Preservative: Bronidox (2ml/L).
Negative Control 1 vial (1.0ml). Inactivated Human serum without HBsAg. Preservative: Bronidox (2ml/L).
Conjugate 1 vial (6ml). The solution contains HRP-labeled anti-HBs, with PBS(0.01mol/L) and stabilizing proteins, ready to use.
5. Sample Diluent 1 vial (3.5ml).

Contains stabilizing proteins and detergent, Preservative: Bronidox (2ml/L).

Wash Buffer (25´) 1 vial (20ml). Diluted 25-fold in distilled water as described in section of Preparation of Reagent.
Substrate Solution A 1 vial (6ml). Hydrogen peroxide-urea (approx. 0.6g/L) in citrate-acetate buffer solution (10 mmol/L).
Substrate Solution B 1 vial (6ml). Tetramethyl benzidine dihydrochloride (10g/L).
Stop Solution 1 vial (6ml). 2N sulphuric acid.
Plate Covers 2 pieces
Instruction manual 1 copy